Transient Induction of Plant Regeneration

PROJECTINFORMATIE
Projectnummer: 1605-045
Thema: Duurzame Plantaardige Productie
Looptijd: 2017- 2020                    
Budget publiek: € - 
Budget privaat:  € - 
Projectleider: Iris Heidmann / e-mail: i.heidmann@enzazaden.nl
Kennisinstelling: DLO/PRI, Bioscience Wageningen UR and Laboratory for Molecular Biology

SAMENVATTING
In vitro embryogenesis techniques are routinely applied in plant breeding programs to clonally propagate material (somatic embryogenesis) or as a fast route to generate homozygous lines (doubled-haploid production). Species and genotype recalcitrance for in vitro embryogenesis is the major bottleneck limiting the use of these biotechnology tools for crop breeding and production. Tissue culture recalcitrance is overcome by a trial and error approach in which the different combinations of explant, growth regulators and culture conditions are evaluated; however empirical identification of the different parameters that contribute to efficient regeneration is time consuming and inefficient, as only a few parameters can be tested at one time.

In this project we will examine the extent to which the LEC1 and BBM transcription factors can be used to transiently promote in vitro regeneration without genomic integration of nucleic acids and without genomic DNA mutation. We will use three approaches to transiently boost LEC1/BBM protein in plant cells: 1) introduction of LEC1/BBM protein with cell penetrating peptides; 2) activation of endogenous LEC/BBM gene expression using CRISPR-dCas9 technology and 3) activation of endogenous BBM/LEC1 gene expression using small compounds. We will focus on improving two types of in vitro regeneration: haploid embryo induction for doubled-haploid production and somatic embryogenesis for clonal propagation. This project will deliver transient, and in part, non-GM methods for improving plant regeneration in crop and model plants.

VOORTGANG 2018
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